A Text-book of bacteriology

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Appleton, 1914 - 766 pages

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Page 532 - Carefully sterilize the syringe after injecting each horse by naming the needle over an alcohol lamp or, better, use separate syringes for healthy and suspected animals. If the same syringe is used, inject the healthy animals first, and flame the needle of the syringe after each injection. "Take the temperature every two hours for at least eighteen hours after the injection.
Page 389 - Distribution. — The colon bacillus is a constant inhabitant of the intestinal canal of human beings and animals. It is also found occasionally in soil, in air, in water, and in milk and is practically ubiquitous in all neighborhoods which are thickly inhabited. When found in nature its presence is generally taken to be an indication of contamination from human or animal sources. Thus, when found in water or milk, much hygienic importance is attached to it.
Page 81 - We have adopted the following scale for making dilutions : For dilutions up to 1 : 70, increase or decrease by a difference of 5 (ie, 5 parts of water). From 1 : 70 to 1 : 160 by a difference of 10. From 1 : 160 to 1 : 200 by a difference of 20.
Page 338 - Preservation at low temperatures (1 to 2 C.) in the ice chest considerably prolongs the life of cultures. Virulence is preserved longest by frequent transplantation upon albuminous media. In sputum or animal excreta, streptococci may remain alive for several weeks. Streptococci are killed by exposure to a temperature of 54 C. for ten minutes.2 Low temperatures, and even freezing, do not destroy some races.
Page 5 - Not to take authority when I can have facts, not to guess when I can know, and not to think a man must take physic because he is sick.
Page 187 - It is plain, therefore, that the animal body must possess more subtle means of defense, by virtue of which pathogenic germs are, even after their entrance into the tissues and fluids, disposed of, or at least prevented from proliferating and elaborating their poisons. The power which enables the body to accomplish this is spoken of as resistance. When this resistance, which in some degree is common to all members of the animal kingdom, is especially marked, it is spoken of as "immunity.
Page 283 - These are pipetted off with a capillary pipette (by careful superficial scratching movements over the surface of the buffy coat). "There being, of course, no absolute scale for phagocytosis, whenever an opsonin determination is made upon an unknown serum, a parallel control test must be made upon a normal serum. This normal is best obtained by a "pool" or mixture of the sera of five or six supposedly normal individuals.
Page 72 - ... is directly applied by means of a gas flame. In the more elaborate stationary devices, steam may be let in by piping it from the regular supply used for heating purposes. Exposure to steam under fifteen pounds pressure (fifteen in addition to the usual atmospheric pressure of fifteen pounds to the square inch) for fifteen to twenty minutes, is sufficient to kill all forms of bacterial life, including spores.
Page 532 - The directions which have been sent out with the mallein, to serve as a guide in testing it, are as follows: Make the test, if possible, with a healthy horse as well as with one or more affected with glanders. Take the temperature of all these animals three times a day for one or two days before making the injection. On the day of making the injection take the temperature every two hours from early in the morning until late in the evening. Use for each horse one cubic centimeter of the solution...
Page 493 - Begin to take the rectal temperature at 6 am, and take it every hour thereafter until midnight. (2) Make the injection at midnight. (3) Begin to take the temperature next morning at 6 o'clock, and continue as on preceding day. To those who have large herds to examine or who are unable to give the time required by the above directions, the following shortened course is recommended: (1) Begin to take the temperature at 8 am, and continue every two hours until 10 pm (omitting at 8...

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