Adenovirus - methods and protocols, volume 1

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William S. M. Wold, Ann E. Tollefson
Springer Science & Business Media, 2007 - Science - 241 pages
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Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
 

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Contents

Manipulation of Early Region 4
2
CoImmunoprecipitation of Protein Complexes
8
The Use of Cell Microinjection for the In Vivo Analysis of Viral
12
Synthesis and Assay of Recombinant Adenovirus Protease
18
Isolation Growth and Purification of Defective Adenovirus
19
Construction of Adenovirus Type 5 Early Region 1 and 4
29
Construction of Mouse Adenovirus Type 1 Mutants
41
Generation of Recombinant Adenovirus Using the Escherichia coli
61
TemperatureSensitive ReplicationCompetent Adenovirus shRNA
125
Evaluating Apoptosis in Tumor Cells Infected With Adenovirus
135
Immunocompetent SemiPermissive Cotton Rat Tumor Model
157
Use of the Syrian Hamster as an Animal Model for Oncolytic
169
A RealTime PCR Method to Rapidly Titer Adenovirus Stocks
185
Detection and Quantitation of Subgroup C Adenovirus
193
Flow Cytometric Detection of Adenoviruses and Intracellular
205
Capture ELISA Quantitation of Mouse Adenovirus Type 1
215

Production and Release Testing of Ovine Atadenovirus Vectors
69
Construction of CapsidModified Recombinant Bovine Adenovirus
91
Fiber Terminal Exon InsertionsGene
107
Preparation and Titration of CsClBanded Adenovirus Stocks
223
Index
237
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