Adhesion Protein Protocols
Amanda S. Coutts
Springer Science & Business Media, 2007 - Science - 234 pages
The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
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acid actin adherent cells agarose alignment aliquots allows analysis antibody approx assay bleaching buffer cadherin calpain calpain activity cell adhesion Cell Biol cell body cell culture cell lines cell migration cell–cell adhesion cellular centrifuge chamber chemokine chemotaxis cleavage collagen complex concentration cover slips culture dish cytoskeleton database diluted DMEM domain dynamics edited endothelial cells extracellular matrix fibroblasts fibronectin filter fluorescent protein focal adhesions fraction FRAP FRAP experiments function gene genomes gradient green fluorescent protein homogenizer HUVEC images Incubate integrin interactions Invitrogen iodixanol leukocytes medium Methods mg/mL microarray microscope microslides microwells Molecular monolayer neutrophils Note peptide photobleaching pipet plasma membrane plate polymerization pseudopodia purified pyrene actin reagent receptors recovery rinse room temperature sample sequence Sigma signaling siRNAs solution store at 20°C Subheading substrate subunits surface time-lapse tion tissue transfection Transwell trypsin tube wash Western blot