Adrenergic Receptor Protocols
Curtis A. Machida
Springer Science & Business Media, 2000 - Medical - 577 pages
In Adrenegic Receptor Protocols, Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, protein expression, transactivator, and second messenger methodologies, as well as immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. Each of the experts writing here details the use of their chosen method in examining the adrenergic receptor system, using aspects of the genetic flow of information as a guide: DNA ' RNA ' transactivator ' protein expression ' second messenger analyses ' cellular analyses ' transgenic whole animal approaches. Comprehensive and rich in practical detail, Adrenergic Receptor Protocols provides the first collection of reproducible methods for the study of these important regulators of CNS-mediated behavior and neural function. Its state-of-the-art methods constitute today's gold standard reference for all neurobiologists, neurochemists, neurologists, and pharmacologists studying this exceptionally important class of receptors.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Other editions - View all
acetate acid activity adenylyl cyclase adrenergic receptor agarose agonist aliquots alprenolol Amersham amplification analysis antigen antisense RNA assay binding Biol blot brain buffer cDNA cell lines centrifuge Chem cloning concentration containing culture denatured detection dilution EDTA electrophoresis enzyme ethanol expression extract filter fragment G-protein G-protein-coupled receptors Genomic human hybridization Incubate kinase labeling ligand medium membrane mg/mL Molecular Biology mRNA NaCl nitrocellulose nonspecific Note nucleotide ODNs oligonucleotide PCR product pellet peptide phage Pharmacol phosphate photoaffinity labels pipet plasmid plate polymerase prepared primary antibody primer probe procedure protein purified radioligand reaction reagents receptor gene receptor subtypes recombinant Resuspend Rinse RNase room temperature sample secondary antibody sections sequence serum slides sodium solution specific step sterile stored Subheading supernatant target template tion tissue transcription transfection transfer Tris-HCl tube vector volume wash Western blotting