Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design
Alexander P. Demchenko
Springer Science & Business Media, Sep 8, 2010 - Science - 390 pages
Fluorescence reporter is the key element of any sensing or imaging technology. Its optimal choice and implementation is very important for increasing the sensitivity, precision, multiplexing power, and also the spectral, temporal, and spatial reso- tion in different methods of research and practical analysis. Therefore, design of ?uorescence reporters with advanced properties is one of the most important problems. In this volume, top experts in this ?eld provide advanced knowledge on the design and properties of ?uorescent dyes. Organic dyes were the ?rst ?uorescent materials used for analytical purposes, and we observe that they retain their leading positions against strong competition of new materials – conjugated polymers, semiconductor nanocrystals, and metal chelating complexes. Recently, molecular and cellular biology got a valuable tool of organic ?uorophores synt- sized by cell machinery and incorporated into green ?uorescent protein and its analogs. Demands of various ?uorescence techniques operating in spectral, anisotropy, and time domains require focused design of ?uorescence reporters well adapted to these techniques. Near-IR spectral range becomes more and more attractive for various applications, and new dyes emitting in this range are strongly requested. Two-photonic ?uorescence has become one of the major tools in bioimaging, and ?uorescence reporters well adapted to this technique are in urgent need. These problems cannot be solved without the knowledge of fundamental principles of dye design and of physical phenomena behind their ?uorescence response.
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Advanced Fluorescence Reporters in Chemistry and Biology III: Applications ...
Alexander P. Demchenko
No preview available - 2011
absorption acceptor acid acridone amino anionic anisotropy applications AvGFP band bathochromic shift binding biological BODIPY cells changes charge transfer Chem Chem Phys chemical chemistry chromophore cm└1 CMe2 compounds conjugation coumarin cyanine cyanine dyes Demchenko AP derivatives detection DMABN donor dynamics effect electric field electron transfer energy transfer ESIPT ESPT excited excited-state fluores fluorescein fluorescence emission fluorescence intensity fluorescence lifetime fluorescence quantum yield Fluorescence Reporters fluorescent labels fluorescent probes fluorescent protein fluorophores green fluorescent protein H-bonding hydrogen bonding increase inhomogeneous broadening interactions intramolecular JAm Chem Soc Lett lipid measured mechanism membrane moiety molecular rotors molecules nonlinear optical oxo-squaraines p-conjugation photophysical properties Phys Chem polymethine potential proton transfer quantum yield quenching red-shifted relaxation Rhodamine rotation sensitive sensor solutions solvation solvatochromism solvent polarity spectroscopy spectrum squaraine dyes Stokes shift structure studies synthesis tautomer TICT transition two-photon viscosity wavelength Z-scan