Advanced Optical Flow Cytometry: Methods and Disease Diagnoses

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Valery V. Tuchin
John Wiley & Sons, Mar 31, 2011 - Science - 734 pages
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A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions.
This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.

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Live Cell Imaging and Cytometry
List of Contributors
Perspectives in Cytometry
Novel Concepts andRequirements in Cytometry
Optics ofWhite
Laser Diffraction by the Erythrocytes
3Parametersof theEktacytometer
Human Research Acknowledgments
Advancesin FluorescenceBased In vivo Flow
In vivo Photothermal and Photoacoustic Flow
Optical Instrumentation for the Measurementof
3Nailfold Capillaroscopy 18 4 LDPMLDPI

Coordinates ofthe DiscCenter 6 8 Light Diffraction by an Ensemble of Particles
Comparisonof Immunophenotyping and Rare
bySFMand FCMUsingQdotStaining 9 3 Comparisonof LeukocyteSubtypingby Multiparametric Analysis with LSC and
Propidium Iodide
Microfluidic Flow Cytometry Advancements
LabelFree Cell Classification with Diffraction Imaging Flow Cytometer
Acknowledgments References
An Integrative
Blood Flow Cytometry andCell Aggregation
Modifications of Optical Properties of Blood
versus In vivo

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About the author (2011)

Valery Tuchin is Head of Chair of Optics and Biomedical Physics and Director of Research-Educational Institute of Optics and Biophotonics at Saratov State University. He has authored more than 250 papers and books, including his latest, Tissue Optics. Light Scattering Methods and Instrumentation for Medical Diagnosis (SPIE Tutorial Texts in Optical Engineering, Vol. TT38, 2000; second edition, PM166, 2007), Handbook of Optical Biomedical Diagnostics (SPIE Press, Vol. PM107, 2002), Coherent-Domain Optical Methods for Biomedical Diagnostics, Environmental and Material Science, Kluwer Academic Publishers, Boston, USA, vols. 1 & 2, 2004, Optical Clearing of Tissues and Blood (SPIE Press, Vol. PM154, 2005), and Optical Polarization in Biomedical Applications (co-authors L. Wang and D.A. Zimnyakov; Springer, 2006).

Some of the contributors:
Martin Leahy, University of Limerick, Ireland
Attila Tarnok, University of Leipzig, Germany
Andreas O.H. Gerstner, University of Bonn, Germany
Anja Mittag, University of Leipzig, Germany
Megha Makam, Daisy Diaz, Rabindra Tirouvanziam,
Stanford University School of Medicine, USA
Steven Boutrus, Derrick Hwu & Cherry Greiner, Tufts University, MA, USA
Michael Chan & Charlotte Kuperwasser, Tufts-New England Medical Center, MA, USA
Charles P. Lin & Irene Georgakoudi, Harvard Medical School,MA, USA
E.I. Galanzha, Saratov State University, Russia
V.P. Zharov, Arkansas University of Medical Science, USA
A.V. Priezzhev, A.G. Lugovtsov, S.Yu. Nikitin & Yu.I. Gurfinkel, Moscow State University, Russia
Valeri P. Maltsev, Maxim A. Yurkin & Elena Eremina,Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia
Alfons G. Hoekstra & Thomas Wriedt,University of Amsterdam, The Neverlands
Peter Nagy % Gyorgy Vereb, Janos Szollsi, University of Debrecen, Hungary

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