Affinity labelling and cloning of steroid and thyroid hormone receptors
The development of powerful affinity labelling techniques has greatly simplified the identification and isolation of intact receptors. The first section of this book offers detailed experimental descriptions of these techniques and those used in conjunction with crosslinking, both methods providing a means of converting reversible into covalent ligand binding. The results obtained in applying these methods to study steroid and thyroid hormone receptors are depicted in the subsequent section whereas the concluding part of the book is devoted to the cloning and functional analysis of these receptors. Each of the seventeen contributions contains comprehensive information on the experimental technique implemented, making the book also useful as a laboratory manual.
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Electrophilic affinity labelling of cellfree nonreceptor molecules
The chicken progesterone receptor
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Acad activity affinity labeling amino acid amino acid sequence analysis androgen receptor antibodies antiestrogen antiglucocorticoid assay aziridine binding domain Biochem Biochemistry Biol buffer c-erbA cDNA Chambon Chem chick oviduct chicken progesterone receptor chromatography column complexes containing covalently labeled crosslinking cytosol deletion dexamethasone digestion DNA binding DNA-cellulose erbA estradiol estrogen receptor etal fractions fragments Gehring glucocorticoid receptor gradient Gronemeyer growth hormone homology hormone binding HTC cell human breast cancer human estrogen receptor human glucocorticoid receptor incubated irradiated Katzenellenbogen Krust lanes ligand Miesfeld molecular weight mouse mRNA mutants Natl nuclear extracts nuclear receptor oviduct pellet peptides photoaffinity labeling precipitated probe progesterone receptor progesterone receptor form protease protein proteolysis purification receptor cDNA region S. S. Jr samples Samuels SDS-polyacrylamide gel specific steroid hormone receptors steroid receptors structure studies synthesis Tam-Az thyroid hormone receptor transcription transfected unactivated vitro wild-type