Agrobacterium Protocols Volume 2

Front Cover
Kan Wang
Springer Science & Business Media, 2006 - Science - 487 pages
Rapid changes and significant progress have been made in the use of Agrobacterium to genetically transform plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Second Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail their best techniques for delivering DNA to plant cells and permanently altering their genomes. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction, major components of plant transformation that are often neglected by many plant biologists. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Agrobacterium Protocols, Second Edition, Volumes 1 and 2 offers plant biotechnologists a gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement, and for inspiration in developing new methods for other related and non-related plants.
 

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Contents

Carrot Daucus carota L
3
Cassava Manihot esculenta Crantz
13
Potato Solanum tuberosum L
25
Sweet Potato Ipomoea batatas L Lam
37
Bermudagrass Cynodon spp
47
Perennial Ryegrass Lolium perenne L
55
Switchgrass Panicum virgatum L
65
Tall Fescue Festuca arundinacea Schreb
75
Apple Malus domestica
253
Blueberry Vaccinium corymbosum L
263
Grapevine Vitis vinifera L
273
Strawberry Fragaria ananassa
287
Walnut Juglans
297
Carnation Dianthus caryophylus L
311
Chrysanthemum Dendranthema grandiflora
321
Orchids Cymbidium spp Oncidium and Phalaenopsis
331

Turf Grasses
83
American Elm Ulmus americana
99
Cork Oak Trees Quercus suber L
113
Eucalyptus
125
Pine Pinus radiata
135
Poplar Populus spp
143
Rubber Tree Hevea brasiliensis Muell Arg
153
Banana Musa sp
167
Citrus
177
Coffee Coffea sp
191
Papaya Carica papaya L
209
Pineapple Ananas comosus L Merr
219
Sugarcane Saccharum spp
227
American Chestnut Castanea dentata Marsh Borkh
239
Petunia Petunia hybrida
339
Rose Rosa hybrida L
351
Ginseng Panax ginseng
361
Hemp Cannabis sativa L
373
Opium Poppy Papaver somniferum
383
Actinomycetes Streptomyces lividans
395
Filamentous Fungi Magnaporthe grisea and Fusarium oxysporum
403
Green Alga Chlamydomonas reinhardtii
421
Mammalian Cells
435
Mushroom Agaricus bisporus
453
Yeast Saccharomyces cerevisiae
465
Index
475
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