Agrobacterium Protocols

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Kevan M. A. Gartland, Michael R. Davey
Springer Science & Business Media, Feb 3, 2008 - Science - 417 pages
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Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants.

Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.
 

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Contents

Growth and Storage of Agrobacterium
1
Simple Cultural Tests for Identification of Agrobacterium Biovars
8
Agrobacterium Virulence Jill S Gartland
15
Agrobacterium tumefaciens Chemotaxis Protocols
29
Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens
37
Binary Ti Plasmid Vectors
47
Leaf Disk Transformation
59
AgrobacteriumMediated Transformation of Protoplasts from Oilseed Rape Brassica napus L
71
The Detection of Neomycin Phosphotransferase Activity in Plant Extracts
201
The Plant Oncogenes rolA B and C from Agrobacterium rhizogenes Effects on Morphology Development and Hormone Metabolism
207
Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells
223
IAA Analysis in Transgenic Plants
237
Quantifying Phytohormones in Transformed Plants
245
Manipulating Photosynthesis in Transgenic Plants
263
Gene Activation by TDNA Tagging
281
Assessing Cadmium Partitioning in Transgenic Plants
295

AgrobacteriumMediated Transformation of Stem Disks from Oilseed Rape Brassica napus L
79
Peanut Transformation
87
Transformation of Soybean Glycine max via Agrobacterium tumefaciens and Analysis of Transformed Plants
101
AgrobacteriumMediated Transformation of Potato Genotypes
121
AgrobacteriumMediated Transformation of Soft Fruit Rubus Ribes and Fragaria
129
HighFrequency and Efficient AgrobacteriumMediated Transformation of Arabidopsis thaliana Ecotypes C24 and Landsberg erecta Using Agrobacter...
135
Transformation Protocols for Broadleaved Trees
149
AgrobacteriumMediated Antibiotic Resistance for Selection of Somatic Hybrids The Genus Lycopersicon as a Model System
167
Histochemical GUS Analysis
185
Fluorometric GUS Analysis for Transformed Plant Material
195
Overexpression of Chloroplastic CuZn Superoxide Dismutase in Plants
309
Agroinfection
325
TDNA Transfer to Maize Plants
343
Use of Cosmid Libraries in Plant Transformations
351
Regulation of Agrobacterium Gene Manipulation
369
How to Assess the Risk of Outcrossing to Wild Relatives
393
Electroporation Protocols for Agrobacterium
405
Index
413
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