Allozyme Electrophoresis: A Handbook for Animal Systematics and Population Studies
Academic Press, 1986 - Science - 410 pages
Inherited enzyme variations, studied using electrophoresis, can be used as markers for the identification of individuals, population structure analysis, the delineation of species boundaries and phytogenetic reconstruction. The purpose of this book is to describe, in a single convenient handbook, all the theoretical and practical matters relevant to those intending to use electrophoresis as a tool for answering such questions.
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acid activity allele frequencies allelic variation allozyme electrophoresis analysis ancestral BACKGROUND INFORMATION Quaternary bands Cathode Duration cell cellulose acetate Chapter character dehydrogenase detected dimer distance divergence Dry component Duration of run E.C. NUMBER electromorphs electrophoretic ELECTROPHORETIC CONDITIONS Buffers electrophoretic data enzyme example exhibit uninterpretable variation figure fixed differences gene genetic markers Genetic phenomena genotype geographical groups haploid Hardy Weinberg Equilibrium Harris and Hopkinson heteropolymers heterozygotes homogenates hybrid individuals INFORMATION Quaternary structure interpretation isozymes Liable to exhibit Linking enzyme loading locus method mobility monophyletic NADP Non-genetic phenomena null alleles null hypothesis Number of loci outgroup paraphyletic patterns phenotypes phylogenetic polymorphism Position of origin possible problems protein Quaternary structure reagents scoring single locus Special comments species Stain buffer STAINING Mechanism Stock solutions sub-bands subpopulations substrate SUGGESTED ELECTROPHORETIC CONDITIONS synapomorphy taxa Taxon tissue Tris-HCl pH8.0 uninterpretable variation note variation note 17 vertebrates warping XI XI