Alterations of the Sister Chromatid Exchange Frequency in Peripheral Lymphocytes Caused by an Ironman Triathlon
GRIN Verlag, 2008 - 94 pages
Diploma Thesis from the year 2007 in the subject Medicine - Human Genetics, grade: 1,0, University of Vienna (Institut fur Ernahrungswissenschaften), 96 entries in the bibliography, language: English, abstract: The aim of the present study was to investigate the effect of a triathlon (3.8 km swim, 180 km cycle, 42,2 km run) on the genomic stability of nine highly trained non- professional athletes. Therefore, the SCE assay, a relevant biological response marker for genotoxicity in human biomonitoring studies [PENDZICH et al., 1997] was performed using peripheral lymphocytes, on account of their effortless accessibility [WILKOSCY and RYNARD, 1990]. Duplicate lymphocyte cell cultures, of each participant, were incubated for 72 h (37 C, 5% CO2) according to a short-term human lymphocyte cell culture. For each participant at least 50 metaphases, containing 43-46 chromosomes were scored, to evaluate the mean SCE frequency. The number of SCEs per cell was calculated to a chromosome set of a normal diploid human cell, containing 46 chromosomes. In the present study the alteration of SCE frequency, 48 h pre- and 24 h postrace was evaluated. As an additional endpoint Top 5 HFCs (highest five absolute SCE means) were assayed. It could be demonstrated that both the total mean SCE frequency and the mean Top 5 HFC frequency (n=9) 24 h postrace were significantly decreased (*p
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MATERIALS AND METHODS
RESULTS AND DISCUSSION
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Alterations of the Sister Chromatid Exchange frequency in peripheral ...
Limited preview - 2008
24 h postrace 48 h prerace adaptive responses Additionally antioxidant AUDESIRK bidest body mass index BrdU CARRANO and NATARAJAN cell cycle centrifuged 1000 rpm changes 48 h colchicine Comet assay compared to 48 correlation cycling training cytogenetic cytogenetic damage decreased DNA damage endurance exercise exercise-induced DNA damage Giemsa h postrace Figure h prerace 24 heparinized HFC frequencies highest five human lymphocytes increased incubated Ironman triathlon MASTALOUDIS mean abs mean Top metaphase micronucleus mitosis NIESS oxidative damage oxidative DNA damage oxidative stress oxygen physical exercise POULSEN prerace 24 h prerace values RADAK relative SCE changes repair replication SCE assay SCE frequency SCE values SCE-cohort n=9 SCEs Abs.SCEs Top SCEs per cell SCEs/cell SIGMA significantly single bout sister chromatid exchange staining strenuous Top 5 HFCs Top 5 HFCs/cell training per week training time h triathlon race TSAI URSO and CLARKSON week km weekly exercise training WILCOSKY and RYNARD WILSON and THOMPSON