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acid acrolein activity aldehydes alternatives amphiphiles animal experiments animal welfare applied assay assessment astrocytes astroglial bilayer Biochemical Biological bovine bronchial epithelial cells budesonide carbachol carcinogenesis carcinogens CCl4 cell cultures cell lines cellular chapter chemicals cholinergic compounds concentration cornea cytotoxicity data bank dexamethasone differentiation dose drugs ECMA effects embryo endothelial enzymes erythrocytes ethanol evaluation experimental explant cultures explants exposure eye irritation fetal fibres fibroblasts Figure genetic glucocorticoids growth guidelines HEPATOCYTES human bronchial increase incubation induced inhibition interactions INVITTOX involved isolated Journal laboratory animals LD50 values lipids liver measured membrane metabolism methods methylmercury Michael Balls monocytes monolayer mouse Mutagenesis MUTAGENICITY Mutation Research neuronal number of animals opacity P.O. Box pain Pharmacology phosphatidylethanol phospholipase porphyrin potency potential primary cultures procedure protein rat brain reaggregate cultures receptor scientists serum species stimulation studies techniques tissue culture toxicity testing uptake vaccines validation vitro toxicology vivo