Alzheimer's Disease: Methods and Protocols

Front Cover
Springer Science & Business Media, 2000 - Medical - 408 pages
0 Reviews
With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of key techniques for the analysis of DNA and protein from patients suspected to suffer from muscular dystrophy. Each method is highly detailed to ensure success and is presented by a hands-on expert who uses it on a day-to-day basis. The various DNA techniques focus on both the X-linked muscular dystrophies and the autosomal recessive muscular dystrophies. The protein methods include expression analysis, multiplex western blot analysis, immunocytochemical analysis, and reviews of immunological reagants and of amplification systems. Also discussed are the use of animal models to understand human muscular dystrophy and the available options for gene-based therapy. Comprehensive and highly practical, Muscular Dystrophy: Methods and Protocols offers today, s diagnostic laboratories, basic and medical researchers, and active clinicians an authoritative collection of tools that will serve as exacting diagnostic tools as well as greatly empowering research on the novel therapeutics now beginning to emerge
 

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

Introduction to Alzheimers Disease David Allsop 1 Introduction
1
The Genetics of Alzheimers Disease Nick Brindle and Peter St GeorgeHyslop
23
Advances in Methodology and Current Prospects for Primary Drug Therapies for Alzheimers Disease David S Knopman
45
Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein APP sAPPα Steven W Barger 1 Introduction
63
Quantifying Aβ140 and Aβ142 Using SandwichELISA Daniel M Skovronsky Jun Wang Virginia MY Lee and Robert W Doms
79
Electrophoretic Separation and Immunoblotting of Aβ140 and Aβ142 Matthias Staufenbiel and Paolo A Paganetti 1 Introduction
91
AβInduced Proinflammatory Cytokine Release from Differentiated Human THP1 Monocytes Kurt R Brunden June KocsisAngle Paula Embury and S...
101
Effects of the βAmyloid Peptide on Membrane Ion Permeability Hugh A Pearson 1 Introduction
113
Using γSecretase Inhibitors to Distinguish the Generation of the Aβ Peptides Terminating at Val40 and Ala42 Paolo A Paganetti and Matthias Staufen...
239
Designing Animal Models of Alzheimers Disease with Amyloid Precursor Protein APPTransgenes Jeanne F Loring 1 Introduction
249
Phosphorylation of Amyloid Precursor Protein APP Family Proteins Toshiharu Suzuki Kanae Ando Koichi Iijima Shinobu Oguchi and Shizu Takeda
271
Determining the Transmembrane Topology of the Presenilins GopalThinakaran and Andrew Doan 1 Introduction
283
Normal Proteolytic Processing of the Presenilins Henrike Hartmann and Bruce AYankner 1 Introduction
297
Apoptotic Proteolytic Cleavage of the Presenilins by Caspases TaeWan Kim 1 Introduction
309
The Phosphorylation of Presenilin Proteins Jochen Walter 1 Introduction
317
Interaction of the Presenilins with the Amyloid Precursor Protein APP Andreas Weidemann Krzysztof Paliga Ulrike Dürrwang Friedrich Reinhard Dai ...
333

Analysis of βAmyloid Peptide Degradation In Vitro Barbara Cordell and Asha Naidu 1 Introduction
139
Posttranslational Modifications of Amyloid Precursor Protein Ectodomain Phosphorylation and Sulfation Jochen Walter and Christian Haass
149
Posttranslational Modifications of the Amyloid Precursor Protein Glycosylation Chen LiuTomasz Rozmyslowicz Magda StworaWojczyk Boguslaw ...
169
Using an Amyloid Precursor Protein APP Reporter to Characterize αSecretase Susan Boseman Roberts 1 Introduction
191
Inhibition of αSecretase by Zinc Metalloproteinase Inhibitors S Parvathy Anthony JTurner and Nigel M Hooper 1 Introduction
203
Development of Neoepitope Antibodies Against the βSecretase Cleavage Site in the Amyloid Precursor Protein Carol W Gray and Eric H Karran 1 In...
217
βSecretase Tissue Culture Studies of Sequence Specificity Inhibitors and Candidate Enzymes Martin Citron
229
Distribution of Presenilins and Amyloid Precursor Protein APP in DetergentInsoluble Membrane Domains Edward T Parkin Anthony JTurner and Ni...
345
Characterization and Use of Monoclonal Antibodies to Tau and Paired Helical Filament Tau Peter Davies 1 Introduction
361
Tau Phosphorylation Both In Vitro and in Cells C Hugh Reynolds Graham M Gibb and Simon Lovestone 1 Introduction
375
TransglutaminaseCatalyzed Formation of AlzheimerLike Insoluble Complexes from Recombinant Tau Brian J Balin and Denah M Appelt 1 Introduct...
395
Index
404
Copyright

Other editions - View all

Common terms and phrases

References to this book

Bibliographic information