Alzheimer's Disease: Methods and Protocols
With the molecular mechanisms underlying the various forms of muscular dystrophy now rapidly clarifying, precise diagnosis has become a reality, and even a requirement in clinical practice. In Muscular Dystrophy: Methods and Protocols, Katherine Bushby and Louise Anderson have assembled an outstanding collection of key techniques for the analysis of DNA and protein from patients suspected to suffer from muscular dystrophy. Each method is highly detailed to ensure success and is presented by a hands-on expert who uses it on a day-to-day basis. The various DNA techniques focus on both the X-linked muscular dystrophies and the autosomal recessive muscular dystrophies. The protein methods include expression analysis, multiplex western blot analysis, immunocytochemical analysis, and reviews of immunological reagants and of amplification systems. Also discussed are the use of animal models to understand human muscular dystrophy and the available options for gene-based therapy. Comprehensive and highly practical, Muscular Dystrophy: Methods and Protocols offers today, s diagnostic laboratories, basic and medical researchers, and active clinicians an authoritative collection of tools that will serve as exacting diagnostic tools as well as greatly empowering research on the novel therapeutics now beginning to emerge
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Acad activity Alzheimer Alzheimer’s disease amino acid amyloid precursor protein analysis analyzed APLP2 apolipoprotein approx assay batimastat Biochem Biol brain C-terminal Ca2+ caspase cDNA cell lines cellular centrifugation channels Chem chromosome cleavage concentration containing dementia described in Subheading detected dilution domain electrophoresis ELISA enzyme epitopes expression familial Alzheimer’s disease forms of APP fragments gene Genet glycosylation human immunoblotting immunoprecipitation Incubate inhibition inhibitors intracellular isoforms lysates medium Methods and Protocols mg/mL molecular monoclonal antibody mouse mutations N-glycans NaCl Natl neurons Neurosci Note patients pellet peptide PHF-tau phosphorylation pipet plate polyclonal presenilin Proc protease protein kinase protein precursor proteolytic processing receptor recombinant sample buffer SDS-PAGE secreted senile plaques sequence Sigma sodium soluble solution specific studies Subheading 3.2 substrate sucrose sulfate supernatant tau protein TGase tissue transfected transgenic transgenic mice Tris-HCl tube vitro vivo voltage wash Western blot Αβ