Amyloid Precursor Protein: A Practical Approach
Weiming Xia, Huaxi Xu
CRC Press, Dec 16, 2004 - Science - 240 pages
In the search for an effective treatment for Alzheimer's disease, APP is a unique model protein that illustrates the wide array of basic and sophisticated characterization techniques available.
Exploring a variety of biological techniques to clarify the structure and function of this transmembrane protein, this text presents each method with detailed, step-by-step protocols to achieve reproducible results and provide a framework for studying other membrane proteins.
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Chapter 2 Assays for Analysis of APP Secretion and Recycling
Chapter 3 Strategies for Crystallizing the NTerminal Growth Factor Domain of Amyloid Precursor Protein
Tools for Memapsin 2 Secretase Inhibition Studies
Chapter 5 Assays for Amyloid Precursor Protein Secretase Activity
Chapter 6 CellFree Reconstitution of Amyloid Production and Trafficking
Chapter 7 Studying Amyloid Protein Assembly
Chapter 8 Intracecellular Accumulation of Amyloid and Mitochondrial Dysfunction in Downs Syndrome
Chapter 10 Regulation of Amyloid Precursor Protein Processing by Lithium
Chapter 11 Immunocytochemical Analysis of Amyloid Precursor Protein and Its Derivatives
Chapter 12 Pathological Detection of A and APP in Brain
Chaptre 13 Creating APP Transgenic Lines in Mice
Chapter 14 Generation of Amyloid Precursor Protein Knockout Mice
A Technical Approach
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Acad activity aggregates Alzheimer Alzheimer’s disease amino acids amyloid beta amyloid precursor protein analysis antibody antigen APP gene aspartic protease assay assembly astrocytes beta beta-amyloid precursor protein binding Biochem Biol biological brain buffer C-terminal cell surface Centrifuge Chem cleavage clones concentration Congo red cultures cytosol detected domain Down’s DS astrocytes ELISA enzyme ethanol expression fibril Figure fluorescence fractions fragment Genet immunoprecipitation Incubate inhibition intracellular Iodixanol labeled lipid medium memapsin membrane method microinjection microscopy mitochondrial molecular monoclonal mouse mutations Natl neurons Neurosci nicastrin normal astrocytes pellet peptide plaques plates prepared presenilin Proc produce protease inhibitor protein precursor proteolytic Protocol recombinant residues resuspended room temperature sample secondary antibody secreted Selkoe sequence serum soluble solution specific staining structure studies substrate supernatant targeting tissue trafficking transfected transgenic transgenic mice transmembrane tube vesicles vitro Wash Western blot wild-type µg/ml