Amyloid Precursor Protein: A Practical Approach

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Weiming Xia, Huaxi Xu
CRC Press, Dec 16, 2004 - Science - 240 pages
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In the search for an effective treatment for Alzheimer's disease, APP is a unique model protein that illustrates the wide array of basic and sophisticated characterization techniques available.

Exploring a variety of biological techniques to clarify the structure and function of this transmembrane protein, this text presents each method with detailed, step-by-step protocols to achieve reproducible results and provide a framework for studying other membrane proteins.
 

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Contents

Chapter 1 Biochemical Characterization of Amyloid Precursor Protein
1
Chapter 2 Assays for Analysis of APP Secretion and Recycling
19
Chapter 3 Strategies for Crystallizing the NTerminal Growth Factor Domain of Amyloid Precursor Protein
29
Tools for Memapsin 2 Secretase Inhibition Studies
41
Chapter 5 Assays for Amyloid Precursor Protein Secretase Activity
51
Chapter 6 CellFree Reconstitution of Amyloid Production and Trafficking
69
Chapter 7 Studying Amyloid Protein Assembly
83
Chapter 8 Intracecellular Accumulation of Amyloid and Mitochondrial Dysfunction in Downs Syndrome
111
Chapter 10 Regulation of Amyloid Precursor Protein Processing by Lithium
145
Chapter 11 Immunocytochemical Analysis of Amyloid Precursor Protein and Its Derivatives
155
Chapter 12 Pathological Detection of A and APP in Brain
165
Chaptre 13 Creating APP Transgenic Lines in Mice
185
Chapter 14 Generation of Amyloid Precursor Protein Knockout Mice
201
Index
219
Back cover
229
Copyright

A Technical Approach
129

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