An Introduction to Metabolic and Cellular Engineering

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World Scientific, 2002 - Science - 248 pages
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Metabolic and cellular engineering, as presented in this book, is a powerful alliance of two technologies: genetics -- molecular biology and fermentation technology. Both are driven by continuous refinement of the basic understanding of metabolism, physiology and cellular biology (growth, division, differentiation), as well as the development of new mathematical modeling techniques. The authors' approach is original in that it integrates several disciplines into a coordinated scheme, i.e. microbial physiology and bioenergetics, thermodynamics and enzyme kinetics, biomathematics and biochemistry, genetics and molecular biology. Thus, it is called a transdisciplinary approach (TDA). The TDA provides the basis for the rational design of microorganisms or cells in a way that has rarely been utilized to its full extent.
 

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Contents

Matter and Energy Balances
39
Cell Growth and Metabolite Production Basic Concepts
77
Methods of Quantitation of Cellular Processes Performance
111
Different Strains of 5 cerevisiae
136
Appendix B
144
Bioprocess Development with Plant Cells
171
Metabolic Compartmentation in Plant Cells
184
MCA Studies in Plants
194
Cellular Engineering
201
Cellular Engineering
212
Bibliography
223
Index
243
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