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Animal Reproduction Laboratory
Care of the Field Liquid Nitrogen Refrigerator
2 other sections not shown
50-ml centrifuge tubes 75°C water bath Animal Reproduction Laboratory artificial insemination B.W. Fertility breeding bull semen cane canister centrifugation at 400xg centrifugation medium Cochran Colorado State University cooling to 5°C cryoprotective diluted semen E.L. and Pickett EDTA ejaculates equine spermatozoa estrus Experiment extender and packaged Fertility of stallion Figure freezing and thawing freezing rate fresh semen frozen semen frozen stallion semen frozen-thawed semen glucose-EDTA solution glycerol immediately after thawing immersed incubation at 37°C insemination doses insemination syringe lactose lactose-EDTA-egg yolk extender liquid nitro liquid nitrogen refrigerator Loomis Marc St mares inseminated matozoa method motility of sperm neck open end pellet percentage of progressively plastic plug plastic sheath plunger polypropylene polyvinyl chloride straw post-thaw motility pregnancy rate progressively motile sperm PVC straws result resuspended semination sper sperm motility sperm/ml Squires stallion semen storage supernatant temperature thawing bath thawing semen Theriogenology transferred uterus warming yolk extender containing