An ultracentrifugal method for the determination of serum lipoproteins
Luigini Del Gatto, Frank T. Lindgren, Alex V. Nichols, University of California, Berkeley. Radiation Laboratory
University of California Radiation Laboratory, 1958 - Medical - 15 pages
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242 seconds analytic cell analytical ultracentrifuge background solution baseline solution Biophysics and Medical California September 29 centrifugal field class of lipoprotein classes of serum component concentration and flotation correspond deLalla final density flotation rates g/ml six determinations Gofman isolated lipoprotein classes Johnston-Ogston correction Johnston-Ogston effect KBr-NaCl Laboratory of Biophysics lipoprotein analysis lipoprotein and protein lipoprotein isolation lipoprotein run lipoprotein spectrum low-density lipoprotein Luigini Del Gatto Medical Physics University medium of density mid-area position milliliters of serum ml of NaBr ml of serum molecular class NaBr medium NaBr solution Nichols Donner Laboratory obtained by planimetry particular lipoprotein class preparative tube preparative ultracentrifugal procedure protein analytical run radial correction rpm for 24 schlieren patterns seconds after starting serum lipoproteins serum proteins simplification solution density solution p?n/4 total lipoprotein typical lipoprotein UCRL-8476 AN ULTRACENTRIFUGAL ultracentrifugal analysis ULTRACENTRIFUGAL METHOD ultracentrifugal rotor speed ultracentrifugal run University of California values for isolated viscosity