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DEVELOPMENT OF THE QUANTITAT1 a RTPCR METHODOLOGY 35
Figure Page 1 MC3T3EI cells
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4-point bend fixture 6-well section agarose gel alignment allowed analysis analyzed axial gage BNC-l bone cells buffer cDNA Cell Tracker cells attached cellular response CMFDA competitor DNA copy number CP-Ti cycles cyclic cytoskeleton DATAQ densitometry dental implants detection oligo evaluated extracellular matrix gene expression HPRT imaged implant materials incubator integrin interaction interface investigation load cell Load vs Hours matrix proteins MC3T3-E1 cells mechanical load mechanical strain metabolic method microliters morphological mRNA oligonucleotide osseointegration osteoblasts osteoblasts seen osteocalcin osteopontin peak strains performed test phalloidin placed plates polymerase polymerase chain reaction Power of performed primer prostaglandin quantified quantitative RT-PCR resulted resuspended rhodamine rhodamine phalloidin stain rinsed RT-PCR strain application strain environment strain gages Strain Magnitude strain protocol strained cells substrate surface template test with alpha Ti-6A1-4V coupons Ti-6A1-4V samples timepoints titanium transverse gage type I collagen vitro vivo