Analytical Techniques

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T.P. Mommsen, Peter W. Hochachka
Newnes, Dec 2, 2012 - Medical - 718 pages
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This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers and students with a pertinent information source from theoretical and experimental angles.
 

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Contents

Chapter 1 Anaesthetics
1
Chapter 2 Cannulation techniques
17
selective islet removal and total pancreatectomy
37
Chapter 4 Hypophysectomy of fish
49
stanniectomy
59
gonadectomy
63
Chapter 7 Hepatectomy
77
Chapter 8 Injection techniques
85
isolation maintenance and utilization
355
Chapter 31 Organ culture of fish tissues
375
Chapter 32 Perifusion of hepatocytes
387
Chapter 33 Measurement of intracellular pH in fish hepatocytes
399
Chapter 34 Measurement of intracellular calcium in fish liver cells
407
designing an in vitro system to study enzymes and metabolism
417
insulin receptor
431
deiodinase and receptor assays
447

Chapter 9 Techniques for recording from electric organs
93
Chapter 10 Intact and demembranated muscle fibres
107
Chapter 11 Isolated perfused fish hearts
119
Chapter 12 Methods for assessing kidney and urinary bladder function in fish
127
availability propagation and isolation
145
Chapter 14 Extracorporeal circulation
161
Chapter 15 Perifusion of pancreatic islets
169
Chapter 16 Perfusion of pancreatic endocrine tissue of teleost fish
179
Chapter 17 The isolated rete
191
isolation culture and use
205
the analysis of bioluminescent systems
215
Chapter 20 Opercular membranes and skin
231
Chapter 21 Isolation of gill cells
239
isolation and culture
249
Chapter 23 Primary culture of rainbow trout corpuscles of Stannius
273
Chapter 24 Isolated cardiac myocytes
283
Chapter 25 Oogenesis
291
Chapter 26 Adipose tissue
305
Chapter 27 Enzymes of lipogenesis
313
Chapter 28 Isolation and culture of head kidney macrophages
327
Chapter 29 In vitro culture of fish leukocytes
341
membrane and intracellular preparations
457
Chapter 39 Membrane cell preparation and receptor assay catecholamines
469
Chapter 40 Membrane preparation and receptor assays adenosine
477
Chapter 41 Fish mitochondria
487
Chapter 42 Peroxisomes and peroxisomal βoxidation
503
Chapter 43 Use of membrane vesicle techniques to characterize nutrient transport processes of the teleost gastrointestinal tract
513
a biological model tostudy hormonal regulation of skeletal growth in fish
525
insulin
535
Chapter 46 Radioimmunoassay of fish growth hormone prolactin and somatolactin
545
application to fish gonadotrophin
557
Chapter 48 Developing an ELISA for a model protein vitellogenin
567
Chapter 49 Bolusinjection of radiolabels in the study of steady state glucose metabolism
579
Chapter 50 In vivo nuclear magnetic resonance spectroscopy of aquatic animals
591
Chapter 51 Analysis of enzyme regulation via reversible phosphorylation and enzyme binding interactions with macromolecules
603
Chapter 52 Metabolite transport in fish red blood cells and hepatocytes
615
Chapter 53 Ca2+ transport across plasma membranes
625
Chapter 54 Analyzing phosphatidylinositol cycling and protein kinase C involvement
639
Buffered salt solutions culture media and tissue homogenization buffers
649
Species index
659
Subject index
663
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