Animal Cell Electroporation and Electrofusion Protocols

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Jac A. Nickoloff
Springer Science & Business Media, 1995 - Science - 369 pages
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This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners.
 

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Contents

IV
3
V
29
VI
41
VII
63
VIII
73
IX
83
X
93
XI
115
XVI
161
XVII
167
XVIII
185
XIX
199
XX
225
XXI
239
XXII
245
XXIII
253

XII
123
XIII
133
XIV
141
XV
151
XXIV
295
XXV
365
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Nicholoff, Harvard University, Boston, MA.

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