Animal Cell Electroporation and Electrofusion Protocols
Jac A. Nickoloff
Springer Science & Business Media, 1995 - Science - 369 pages
This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners.
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activity aliquot Animal Cell Electroporation antibody antigen approx assay bilayer Biochem Biol Biophys blastomeres bovine Ca2+ capacitance cell lines cell membrane cell types cell viability centrifugation clones colonies complete ES medium concentration containing culture medium cuvet described dish electric field electrical breakdown electrode electroporation Electroporation and Electrofusion embryos enzyme exponential exponential decay feeder cells fibroblasts Filter-sterilize flask fluorescence fused fusion medium gene transfer growth medium hormone human hybrid hybridomas Incubate Introduction linear luciferase mammalian cells mg/mL molecules monoclonal monoclonal antibodies mouse Nickoloff Humana Press Note number of cells oocytes optimal parameters pipet plasmid plasmid DNA plate pores potential procedure promoter protein quantitation recombination reporter gene resistance resuspend room temperature satellite DNA selection serum Sigma stained sterile stock solution target tion tissue trans transfection transfection efficiency transgenic transient transmembrane trypan blue trypsin tube vector vitro voltage volume wash zona pellucida