Animal Toxins: Facts and Protocols
Hervé Rochat, Marie-France Martin-Eauclaire
Springer Science & Business Media, 2000 - Science - 365 pages
Natural toxins form a major component of the molecular tools used increasingly frequently by the ever growing number of laboratories of various kinds. Evidence for this is provided not only by the increasing number of firms including such toxins in their catalogues but also by the large number of demands received by those who discover new toxins. Twenty chapters survey important aspects of toxin origin, their structure and molecular mechanism, and their cellular and pathogenic effects. In addition, the text provides comprehensive and specific methodology for the application of these toxins in the research laboratory. This begins with the description of the method of extraction, biochemical and pharmacological characterization, and assessment of purity, and continues with methods for chemical modification, e.g. labelling, and eventually describes applications in pharmacological studies in vivo and/or in vitro. The length of this book has been kept reasonable by concentrating on...
What people are saying - Write a review
We haven't found any reviews in the usual places.
Other editions - View all
AChE activity amino acid ammonium acetate anabaseine antibodies apamin assay bee venom Beress binding Biochem Biochemistry Biol block bradykinin buffer Ca2+ cDNA cells centrifugation charybdotoxin Chem chemical chromatography clones column concentration conotoxins containing dendrotoxin dilution effects eluted enzymes equilibrated ex ex ex ex ex factor X fasciculin filters flow rate fraction gradient HPLC incubation inhibition inhibitor interaction iodination isolated K+ channels Kem WR kinins Lazdunski lyophilized mamba Martin-Eauclaire mastoparan MCD peptide melittin membrane method microcystins molecular molecule muscle neuronal neurotoxins nicotinic oxidation peptide peptide resin Pharmacol pharmacological phospholipase A2 PLA2 enzymes platelet polypeptides potassium channels potential preparation protein Protocol purification radiolabeled rat brain reaction reagent receptor residues Rochat H sample scorpion scorpion toxins sea anemone sea anemone toxins Sephadex Sigma Siigur snake venom sodium channels solution specific structure supernatant synthesis target tion toxic Toxicon toxin trifluoroacetic acid tube wasp