Animal Cell Culture Techniques
Springer Berlin Heidelberg, Jun 16, 1998 - Science - 618 pages
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
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Cloning Animal Cells
Screening for Mycoplasma Contamination
27 other sections not shown
acid activity agar antigen apoptosis assay baculoviral baculovirus buffer Ca2+ Cancer cell culture cell lines cell number cell suspension cell type cells/ml cellular centrifugation cloning colonies concentration containing coverslips culture medium cyclin cytokines detection differentiation dilution DMSO drug EDTA elution endotoxin enzyme epithelial cells ethanol expression fetal filter flask fluorescence gene growth factor growth medium hepatocytes HL-60 cells human infection inoculated inositol inositol phosphates insect cells intracellular isolated lung lysate macrophages membrane method mg/ml microscope monoclonal antibodies monolayer mouse murine mycoplasma NaCl neutrophils pellet petri dishes phosphate pipette plaque plates prepared Procedure protein protocol recombinant remove resuspend ribozyme room temperature sample sensitive serum serum-free Sigma sodium solution specific staining sterile stored supernatant techniques tion tissue culture toxicity transfection trypsin tube tumor type II cells ug/ml vector viral virus viruses vitro volume wash