Antibiotic Resistance: Methods and Protocols

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S. H. Gillespie
Springer Science & Business Media, 2001 - Medical - 287 pages
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At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls. Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.
 

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Contents

Multiplex Polymerase Chain Reaction Detection of vanA vanB vanC1 and vanC23 Genes in Enterococci
3
Drug Susceptibility of Mycobacterium tuberculosis Through the Mycolic Acid Index
13
MicroWell Phage Replication Assay for Screening Mycobacteria for Resistance to Rifampin and Streptomycin
21
Application of SSCP to Identification of Resistance Mutations
31
Quantitative SingleTube Nested PCR QSTNPCR for Determining the Antibiotic Susceptibility of Mycobacterium tuberculosis
39
Rapid Rifamycin Susceptibility Testing of SmallVolume Mycobacterium tuberculosis Cultures by Detection of Precursor rRNA
55
Detection of Penicillin Resistance in Streptococcus pneumoniae by a Seminested PCR Strategy
65
Diagnosis of Penicillin Resistance by PCRRFLP
77
BOX PCR Fingerprinting for Molecular Typing of Streptococcus pneumoniae
159
Restriction Fragment End Labeling Analysis HighResolution Genomic Typing of Streptococcus pneumoniae Isolates
169
Pulsefield Gel Electrophoresis for Epidemiological Studies of Streptococcus pneumoniae
181
Outer Membrane Profiles of Clonally Related Klebsiella pneumoniae
189
Atomic Force Microscopy Theory and Practice in Bacteria Morphostructural Analysis
199
Assessing the Activity of Bacterial Multidrug Efflux Pumps
211
The Use of a Continuous Culture System to Study the Antimicrobial Susceptibility of Bacteria in Biofilm
215
Estimation of Mutation Rates in Antibiotic Research
227

Detection Methods of GlycopeptideResistant Staphylococcus aureus I Susceptibility Testing
85
Detection Methods for GlycopeptideResistant Staphylococcus aureus II Cell Wall Analysis
93
Multiplex PCR for the Rapid Simultaneous Speciation and Detection of MethicillinResistance and Genes Encoding Toxin Production in Staphylococc...
103
Chromogenic Detection of Aminoglycoside Phosphotransferases
113
Quantification of M tuberculosis DNA in Sputum During the Treatment of Pulmonary Tuberculosis
121
Isolation of M tuberculosis RNA from Sputum
133
Detection of Viable Mycobacterium tuberculosis by Reverse TranscriptaseStrand Displacement Amplification of mRNA
141
In Vitro Assessment of the Fitness of Resistant M tuberculosis Bacteria by Competition Assay
233
Purification of DNA Topoisomerases and Inhibition by Fluoroquinolones
237
SiteDirected Mutagenesis to Determine Structure Function Relationships in Streptococcus pneumoniae PenicillinBinding Protein Genes
245
Detection of Low Affinity PenicillinBinding Protein Variants in Streptococcus pneumoniae
265
Mobilization of Transposons Rationale and Techniques for Detection
275
Index
285
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Page 264 - Hemsley, A., Arnheim, N., Toney, MD, Cortopassi, G., and Galas, DJ (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res.

About the author (2001)

Stephen H. Gillespie is Professor of Medical Microbiology in the Department of Infectious Diseases at University College London, UK.

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