Antibody Expression and Production

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Mohamed Al-Rubeai
Springer Science & Business Media, May 16, 2011 - Medical - 346 pages
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Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy.
 

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Contents

1 Expression of Antibody in Mammalian Cells
1
2 Bioreactor Systems for Producing Antibody from Mammalian Cells
25
3 Production of Antibody in Insect Cells
53
4 Production of Monoclonal Antibodies in Glycoengineered Pichia pastoris
77
5 Production of Antibodies in Hansenula polymorpha
99
6 Production of Antibody by Transgenic Avians
121
7 Production of Antibodies in Plants
143
8 Production of Antibody Fab Fragments in Escherichia coli
165
9 Intrabody Expression in Mammalian Cells
179
10 Engineering Antibodies for Cancer Therapy
197
11 Recombinant Bispecific Antibodies for Cancer Therapy
235
12 The Role of Glycosylation in Therapeutic Antibodies
251
13 Quality Issues Arising from Posttranslational Modification of Recombinant Antibodies
293
14 Recovery and Purification of Antibody
305
Subject Index
341
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