Antigen Retrieval Immunohistochemistry Based Research and Diagnostics
Shan-Rong Shi, Clive R. Taylor
John Wiley & Sons, Jan 14, 2011 - Science - 434 pages
The most complete, up-to-date reference on antigen retrieval and immunohistochemistry
An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment.
Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects.
Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work:
Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products.
Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor
What people are saying - Write a review
An excellent, comprehensive and well researched book on immunocytochemistry using simple fixatives and antigen retrieval methods which are easy to perform without expensive or complex equipment. The techniques described are easily followed even by a novice. The method is useful to rapidly determine the antigenic profile of malignant tumors without having to await a biopsy/ excision procedure and histopathological processing of fissue. This method will save about one week of diagnostic turnaround time ,besides providing very fresh tissue for accurate scoring of positive results. The appropriate line of treatment can be arrived at by the clinician very soon.
the protocols for cell block preparation as well antigen retrieval and immunocytochemical staining are laid ouy in a very readable , lucid style, at the same time time paving the road for standardization and validity testing at one's own laboratory.This is a well referenced useful hand book for any developing pathology laboratory with earnest students of pathology and laboratory technology