Apoptosis Methods and Protocols

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Hugh J. M. Brady
Springer Science & Business Media, 2004 - Science - 304 pages
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Apoptosis research has expanded exponentially in the last 5 or 6 years to embrace virtually all areas of biological and medical research. All types of life scientists now find themselves addressing problems relating to apoptosis. Apoptosis Methods and Protocols collates and standardizes all the important techniques for detection, quantification and functional analysis of apoptosis in a wide variety of cell types and contexts. It will be of great value to biochemists, physiologists and molecular biologists whose research impinges upon cell death. It will also interest clinical researchers who are increasingly aware of the contribution of deregulated apoptosis to many disease states from cancer to neurodegenerative disorders.
 

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Contents

Measurement of Apoptosis by DNA Fragmentation
1
Measurement of Caspase Activity in Cells Undergoing Apoptosis
19
Flow CytometryBased Methods for Apoptosis Detection in Lymphoid Cells
31
Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays
43
Detecting and Quantifying Apoptosis in Tissue Sections
67
Immunoassay for SingleStranded DNA in Apoptotic Cells
85
Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptosis
103
Measurement of Changes in Intracellular Calcium During Apoptosis
117
Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons
169
Measuring Programmed Cell Death in Plants
179
Detection of Apoptosis in Drosophila
191
Measurement of Apoptotic Cell Clearance In Vitro
207
Yeast TwoHybrid Screening for Proteins Interacting With the AntiApoptotic Protein A20
223
The Yeast ThreeHybrid System As a Tool to Study Caspases
243
Cloning of ApoptosisRelated Genes by Representational Difference Analysis of cDNA
255
Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis
275

Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2
131
Assays to Measure StressActivated MAPK Activity
145
Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability
157

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