Apoptosis Techniques and Protocols

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Judes Poirier
Humana Press, 1997 - Science - 286 pages
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In Apoptosis Techniques and Protocols leading experts offer investigators at all levels of experience an indispensable introduction to the basics of apoptosis, as well as essential details of the methods used in its study. These authorities cover such important topics as the histological, biological, and molecular criteria for apoptosis and programmed cell death; necrosis and apoptosis in the CNS; and cellular, invertebrate, animal, and human models of apoptosis in Alzheimer's disease, AIDS, and stroke. The techniques they describe examine the critical steps involved in the apoptotic process, and include PCR analysis of cell-cycle-regulated proteins, histochemical analysis of DNA regulation, DNA laddering analysis, and cytochemical alterations of living cells. Apoptosis Techniques and Protocols provides a wide variety of significant methods for both experimental and clinical investigation. It is certain to serve as an illuminating introduction to the basic ideas behind the phenomena of apoptosis and necrosis, as well as a key technical reference on the significant methodologies used in the field.

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