Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

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Awtar Krishan, H. Krishnamurthy, Satish Totey
John Wiley and Sons, May 12, 2011 - Science - 289 pages
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A much-needed primer on the use of laser flow cytometry for stem cell analysis

Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells.

Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction.

Chapter coverage includes:

  • Stem cell analysis and sorting using side population
  • Flow cytometry in the study of proliferation and apoptosis
  • Stem cell biology and application
  • Identification and isolation of very small embryonic-like stem cells from murine and human specimens
  • Hematopoietic stem cells—issues in enumeration
  • Human embryonic stem cells: long-term culture and cardiovascular differentiation
  • Limbal stem cells and corneal regeneration
  • Flow cytometric sorting of spermatogonial stem cells
  • Breast cancer stem cells
  • Stem cell marker expression in cells from body cavity fluids

This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.

 

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Contents

Basics of Flow Cytometry
1
Practical Considerations for Flow Cytometric Sorting of Stem Cells
13
Stem Cell Analysis and Sorting Using Side Population Analysis
25
Flow Cytometry in the Study of Proliferation and Apoptosis
45
Flow Cytometric Analysis of Drug Transport and Efflux in Stem
61
Stem Cell Biology and Application
75
Identification and Isolation of Very Small Embryoniclike Stem Cells
91
Electronic Volume of Hematopoietic Stem Cells
103
LongTerm Culture
161
Mesenchymal Stromal Cells and Their Clinical Applications
175
Circulating Adult Stem Cells of Hematopoietic Origin for Vascular
189
Flow Cytometric Characterization of Neural Progenitors Derived
211
Limbal Stem Cells and Corneal Regeneration
223
Flow Cytometric Sorting of Spermatogonial Stem Cells
241
Breast Cancer Stem Cells
251
Tumor Stem Cell Marker Expression in Cells from Body Cavity
271

Issues in Enumeration
115
Development and Characterization
135

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