Applications of PCR in Mycology

Front Cover
P. D. Bridge
CABI, Jan 1, 1998 - Science - 357 pages
2 Reviews
Polymerase chain reaction (PCR), a powerful method for amplifying DNA fragments, has had an enormous impact on research throughout biology. This book discusses the wide-ranging applications of PCR in pure and applied mycology. Following an overview of PCR techniques, the book contains a series of chapters where renowned mycologists describe the use of PCR in their own specialized fields. Topics include fungal gene expression and cloning, taxonomy and speciation, fungal mycobionts, mycorrhizal fungi, entomopathogenic fungi, mycotoxin-producing fungi, diagnosis of fungal animal infections, seed-borne diseases, fungal/plant interactions and applications with industrially-important fungi. The volume, which concludes with a discussion of future directions for PCR work, is an invaluable source for researchers and advanced students in all branches of mycology and for biologists and biochemists interested in molecular techniques.
 

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Contents

an Overview
1
2 Gene Cloning Using PCR
21
3 DDRTPCR for Screening of Fungal Gene Expression
47
4 Interpretation of PCR Methods for Species Definition
63
5 PCR Applications in Studies of Lichenforming Fungi
85
6 Applications of PCR for Studying the Biodiversity of Mycorrhizal Fungi
107
7 PCR Applications in Fungal Phylogeny
125
8 PCR Applications to the Taxonomy of Entomopathogenic Fungi
153
10 Application of PCR in Studying Lignocellulose Degradation by Basidiomycetes
205
11 PCR Methods for the Detection of Mycotoxinproducing Fungi
243
12 PCR Diagnostics in Medical Mycology
267
13 Applications of PCR in FungalPlant Interactions
289
14 PCR as a Tool for the Investigation of Seedborne Disease
309
15 Future Directions for PCR in Mycology
325
Index
347
Copyright

9 Application of PCR in Fungal Biotechnology
187

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About the author (1998)

P. D. Bridge, CABI Biosciences UK Center, Egham. D. K. Arora, Center for Advanced Study in Botany, Banaras Hindu University. C. A. Reddy, Department of Microbiology, Michigan State University.

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