Applications of Fluorescence in Immunoassays
Describes the basic prerequisities for a fluorometric immunoassay: the antibody, the immunological technology, the fluorescent probe, and the instrument. Provides a profile of the clinical applications of the various assay technologies. Topics include: background noise, the mechanization and automation of immunoassays, DNA-based specific binding assays, fluorescence immonosensors, multiparameter analyzing systems and more.
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4MUP 6-diketone acid Acta albumin ALP-Ab Anal analytes antigens applications assays based automated avidin B-PE background beads Biochem Biol cells Chapter chelates Chem Chim Clin conjugate coupled Cytometry D. S. Smith derivatives detection developed Digoxin Drug E. P. Diamandis ELFIA emission energy transfer enhancement enzyme Eu-Ab Eu-chelates excitation FITC FITC/Ag Bound flow cytometer flow-cytometric fluo fluorescein fluorescence immunoassays fluorescent chelates fluorescent compounds fluorescent probes fluorochromes fluorogenic fluorogenic substrate fluoroimmunoassays fluorometric FPIA haptenic Hemmilä homogeneous assays hybridization assays IFMA FITC-Ab immunoassays Immunol immunological immunoreaction ions labeled antibodies labeled reagent Landon lanthanide laser ligand liposomes Lövgren Luminescence measured Methods microbeads Microbiol molecules monoclonal antibodies particles Patent Appl Phenytoin phosphorescence phycobiliproteins polarization produced proteins quantitative quenching reaction rhodamine samples sensitivity Sep-FIA serum SL-FIA solid-phase specific stability staining Steroid streptavidin surface Table techniques tested theophylline time-resolved fluorometry TR-FIA tracer umbelliferone