Applied Molecular Genetics
This text explains the key biochemical and cell biological principles behind some of today′s most commonly used applications of molecular genetics, using clear terms and well–illustrated flow schemes. The book is divided into several sections and moves from basic to advanced topics while providing a concise overview of fundamental concepts in modern biotechnology. Each chapter concludes with a Laboratory Practicum describing a hypothetical research objective and the sequence of steps that are most often used to investigate biological questions using molecular genetic methods. In addition, the book provides informative summaries of the latest advances in molecular genetics, using attractive illustrations and a comprehensive reference list.
This text also introduces the use of Internet resources through the World Wide Web as a powerful new tool in molecular genetic research. Seven appendices are included in the book, providing a convenient information resource for properties of nucleic acids, protein and restriction enzymes, a description of common E. coli genetic markers and gel electrophoresis parameters, as well as a list of useful Internet address sites.
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BIOCHEMICAL BASIS OF APPLIED
DNA Metabolizing Enzymes
Identifying the transcriptional start site of a gene
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Acad Acids Res activity agarose amino acid amplification analysis antibody applied molecular genetics assay bacterial bacteriophage base pairs biochemical Biol cDNA cloning cDNA library cDNA synthesis cell lines cell-specific chromosome cloning vector coding sequence contain database denatured detection developed digestion DNA binding DNA fragments DNA polymerase DNA sequence DNasel double-strand EcoRI encoding eukaryotic expression vectors Figure function fusion proteins gel electrophoresis gene expression genomic DNA human genome hybridization identify infection insert Internet isolated laboratory ligation marker membrane method mice molecular genetic mouse mRNA mutagenesis mutations Natl Northern blot Nucl nucleic acid nucleotide oligo oligonucleotide PCR products phage plaque plasmid plasmid vector plate primer pairs probe Proc promoter purified reaction receptor recombinant region regulatory replication reporter gene repressor restriction enzyme RNase RT-PCR samples screening specific strand strategy target template transcription factor transfection transgenic vitro vivo yeast