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Replication Studies With BrdU Labeled
Preliminarv Studies of Nitrous Acid
5-bromouracil Aavaan Ad 2 DNA added to infected adenovirus DNA adenovirus particles contained adenovirus type Buoyant density analysis capsids cell line cells were collected centrifuged and collected cesium chloride Chang's liver cells chloride density 1.34 collected and frozen collected by drop collected under identical components CsCl density gradient cytosine DNA Buoyant density DNA Density DNA replication DNA synthesis DNA was extracted frozen until virus gm/ml H thymidine hours post infection incubated infected Chang's liver isoamyl alcohol isolated and purified KB cells label was added later the cells liver cells maintained lOSANCElfj lower band main virus band maintained in medium medium containing thymidine ml cesium chloride Mutagenized mutant nitrous acid nitrous acid mutagenized observed OFCAIIFO P/BrdU peak plaque assay plates protein rebanded rotor semiconservative replication sequence replication study study of adenovirus thesis thymidine titer type 2 adenovirus type 2 DNA upper bands Vero cell Viral Band Pattern virions Virology virus stocks