Aqueous Two-phase Systems: Methods and Protocols
Springer Science & Business Media, 2000 - Science - 440 pages
In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
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affinity ligand affinity partitioning Albertsson amino acids aqueous two-phase systems assay ATPS binodal Biochem Biotechnol Biotechnology bottom phase cell partition centrifugation Chromatogr chromatography column detergent determined Dextran diluted enzyme equilibrated ethanol Eudragit extraction fraction hydrophobicity increase incubation inside-out interactions interface Johansson Kula ligand liquid-liquid lower phase magnetic particles measured membrane vesicles Metal Ions Methods and Protocols mixing mixture molecular weight molecules NaCl obtained partition coefficient Partitioning in Aqueous partitioning of proteins PEG-LH PEG-rich peptides phase components phase composition phase diagram phase separation phase system phase-forming phosphate buffer pipet plasma membranes polyethylene glycol polymer potassium phosphate prepared procedure protein partitioning purification recovery room temperature salt sample Shanbhag Sigma sodium phosphate soluble solvent stock solution Subheading sucrose sulfate surface systems containing thylakoid tie-line tion top and bottom top phase tube two-phase partitioning upper phase vesicles virus volume ratio