Artificial Culture of Marine Seaweeds in Recirculation Aquarium System

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U.S. Naval Radiological Defense Laboratory, 1966 - Culture media (Biology) - 20 pages
Ulva as either sporeling or transplant could be cultured for periods of 3 months in closed recirculating aquarium systems. Early development of Ulva lobata sporelings proceeded normally and rapidly under conditions imposed and compared to closely related species, i.e., Ulva pertusa and Ulva lactuca. A modified Haxo-Sweeney enrichment was used, substituting B vitamins and organic phosphate for soil extract. Continuous flow ultra-violet sterilization and microfiltration were provided. pH was maintained automatically at 7.9 plus or minus 0.3, using tris buffer and gaseous CO2. Improved fluorescent illumination for 13-15 hours favored culture of sporelings and summer transplants. Irradiance was confined to the spectrum lying between approximately 380-725 millimicrons and amounted to 3.7 kilolux. From field observations, photoperiod appeared closely correlated to initiation of vegetative growth during early spring. Water temperature seemed to have a greater effect on the rapid maturation of gametes and zoospores. (Author).

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