Artificial Culture of Marine Seaweeds in Recirculation Aquarium System
U.S. Naval Radiological Defense Laboratory, 1966 - Culture media (Biology) - 20 pages
Ulva as either sporeling or transplant could be cultured for periods of 3 months in closed recirculating aquarium systems. Early development of Ulva lobata sporelings proceeded normally and rapidly under conditions imposed and compared to closely related species, i.e., Ulva pertusa and Ulva lactuca. A modified Haxo-Sweeney enrichment was used, substituting B vitamins and organic phosphate for soil extract. Continuous flow ultra-violet sterilization and microfiltration were provided. pH was maintained automatically at 7.9 plus or minus 0.3, using tris buffer and gaseous CO2. Improved fluorescent illumination for 13-15 hours favored culture of sporelings and summer transplants. Irradiance was confined to the spectrum lying between approximately 380-725 millimicrons and amounted to 3.7 kilolux. From field observations, photoperiod appeared closely correlated to initiation of vegetative growth during early spring. Water temperature seemed to have a greater effect on the rapid maturation of gametes and zoospores. (Author).
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3.7 kilolux additional Algae amounted to 3.7 appeared closely correlated buffer and gaseous cells closely related species compared to closely correlated to initiation CULTURE OF MARINE culture of sporelings cultured for periods Cummins development of Ulva division early spring enrichment Enter expansa field observations Figure gametes and zoospores gametophyte gaseous CO2 greater effect growth during early initiation of vegetative Laboratory lactuca light lying between approximately maintained automatically MARINE SEAWEEDS maturation of gametes Medical months natural Naval normally and rapidly occurred organic phosphate pH was maintained phosphate for soil photoperiod appeared closely plants present range rapid maturation rapidly under conditions recirculating aquarium systems requirements Research rhizoid rt rt salinity sea water seasonal Security Classification soil extract spectrum lying sporeling or transplant sporelings and summer sterilization and microfiltration Strand substituting thalli Title tris buffer Ulva lobata sporelings UNCLASSIFIED vegetative growth Water temperature seemed