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Intracellular Localization of Arginase
RESULTSPART II continued
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0.01 M glycine 20 minutes Acetone Fractionation acetone powder acetone precipitation acrylamide activation mixture aliquot ammonium sulfate Ammonium Sulfate Fractionation arginase activity arginase Results Arginine assay for arginase Bach Bio-Gel Buchner funnel buffer catesbeiana liver cation CD CD cd H Chromatography on DEAE-Cellulose column cytoplasm described under Methods dilute protein solution disc gel electrophoresis eluted enzyme enzyme solution ethanol extract arginase frog liver arginase gel electrophoresis gel filtration glycerol histidine histidine-Cl homogenized i-H cd increase intensity band intracellular localization ION EXCHANGE CHROMATOGRAPHY ions isoelectric points isozymes of arginase liver pigments liver proteins localization of arginase manganese mg/ml mitochondria MnCl2 MnClp NaCl pre-incubation protein protein concentration purification procedure purification schemes Purification Step rat liver arginase Roholt Sakai Sephadex specific activity stabilize arginase supernatant Table temperature Tris-Cl units/ml urea cycle vacuum flask vivo void volume Waring blendor