Assessment of Blue-green Algal Toxins in Raw and Finished Drinking Water

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American Water Works Association, 2001 - Technology & Engineering - 179 pages
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Contents

INTRODUCTION
1
General Diagnosis of a Toxic Cyanobacteria Episode
2
Structure and Activity of Cyanotoxins
3
Anatoxins and Paralytic Shellfish PoisonsThe Neurotoxins
5
Detection of Cyanotoxins
6
Acute and Lethal Exposures to Cyanotoxins
8
Case Report of Human Deaths From Intravenous Exposure to Microcystins
10
Implications for Humans
12
ELISA of Utility Water Samples
36
Procedure for Taste and Odor Testing
38
RESULTS AND DISCUSSION
43
Preliminary Data for Microcystin Analyses
44
EnzymeLinked Immunosorbent Assay Results
45
Generation of Standard Curve
46
HighPerformance Liquid Chromatography Analyses
47
Taste and Odor Results for Utilities
48

Risk Assessment
13
Risk Associated With Drinking Water
14
Calculation of Risk for Microcystins in Drinking Water Supplies
15
Summary
16
METHODS AND MATERIALS
23
Utility Participants
24
Guidance Documents for Utilities
25
Collection and Shipment of Samples by the Utilities
26
Sample Handling Procedure for Wright State University
33
Report Form for Metropolitan Water District of Southern California
34
Procedure for Direct Competitive ELISA
35
SUMMARY AND CONCLUSIONS
103
RECOMMENDATIONS TO UTILITIES
107
LISTING OF UTILITIES
109
GUIDANCE DOCUMENTS FOR UTILITIES
121
PREPROJECT INFORMATION FROM UTILITIES
125
AWWARF ALGAL TOXINS PROJECT ADDRESS LIST
129
MONTHLY LISTING OF MICROCYSTIN BY ELISA PLUS TO RESULTS
133
REFERENCES
171
ABBREVIATIONS
177
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Page 173 - Codd. GA. 1990. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases and 1 and 2A from both mammals and higher plants.

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