Atomic Force Microscopy: Biomedical Methods and Applications

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Pier Carlo Braga, Davide Ricci
Springer Science & Business Media, 2004 - Science - 394 pages
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Provides biomedical researchers the hands-on knowledge for using atomic force microscopy in their research. It offers many benefits including high manification with high resolution, minimal tissue or cell preparation, the ability to obtain different views of the sample from a single data collection, and the ability to study live cells in their physiologic environment. The authors begin with an introduction on the basics of atomic force microscopy and then covers specific protocols for the morpho-structural analysis of cellular structures. Also covered are detailed protocols for investigating subcellular structures and the latest techniques for functional investigations using AFM. The 28 chapters should prove to be an essential laboratory manual for all scientists using atomic force microscopy in their biomedical research.
 

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Contents

How the Atomic Force Microscope Works
3
Imaging Methods in Atomic Force Microscopy
13
Recognizing and Avoiding Artifacts in AFM Imaging
25
Advanced Biosensing Using Micromechanical Cantilever Arrays
39
MORPHOSTRUCTURAL ANALYSIS OF CELLULAR STRUCTURES
51
Analysis of Human Fibroblasts by Atomic Force Microscopy
53
Corneal Tissue Observed by Atomic Force Microscopy
69
AFM Study of Surface Structure Changes in Mouse Spermatozoa Associated With Maturation
85
Atomic Force Microscopy of Protein Complexes
217
Atomic Force Microscopy of Interfacial Monomolecular Films of Pulmonary Surfactant
231
HighResolution Analysis of the 3D Organization of Human Metaphase Chromosomes
245
Shape and Volume of Living AldosteroneSensitive Cells Imaged with the Atomic Force Microscope
255
Localization of Epithelial Sodium Channels by Atomic Force Microscopy
281
HighResolution Imaging of Bacteriorhodopsin by Atomic Force Microscopy
291
FUNCTIONAL INVESTIGATIONS WITH AFM
305
Measurement of Mechanical Properties of Intact Endothelial Cells in Fresh Arteries
307

Calculation of Cuticle Step Heights from AFM Images of Outer Surfaces of Human Hair
95
Imaging Living Chondrocyte Surface Structures With AFM Contact Mode
105
Growth Cones of Living Neurons Probed by Atomic Force Microscopy
125
Evaluating Demineralization and Mechanical Properties of Human Dentin With AFM
141
Applying Atomic Force Microscopy to Studies in Cardiac Physiology
161
Imaging Bacterial Shape Surface and Appendages Before and After Treatments With Antibiotics
179
SUBCELLULAR STRUCTURES INVESTIGATION
189
Visualizing Nuclear Structure In Situ by Atomic Force Microscopy
191
Imaging Surface and Submembranous Structures in Living Cells With the Atomic Force Microscope Notes and Tricks
201
Observation of Oxidative Stress on Yeast Cells
315
Lymphoblastoid Cells Exposed to LowFrequency Magnetic Fields Study by Atomic Force Microscopy
323
Sample Preparation Method for Observing RNA Polymerase Activity by Atomic Force Microscopy
341
Atomic Force Microscopy of βAmyloid Static and Dynamic Studies of Nanostructure and Its Formation
349
How to Build Up Biosensors With the Cantilever of the Atomic Force Microscope
365
Measurement of Single Molecular Interactions by Dynamic Force Microscopy
369
Index
383
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