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staining antigens with electron
Preparation of electron microscope autoradiographs
12 other sections not shown
acetate acid analysis antibody antigens Appendix applied autoradiographs biochemical biological Cell Biol chemicals chemography circle concentration conjugate crystals curve Cytochem cytoplasm dehydration described diameter dipping distilled water distribution electron microscopy embedding emulsion layer enzyme error estimates ethanol experiments exposure ferritin film filter fixation fixative formaldehyde fractions Glauert glutaraldehyde grain counting grain density granule grids Histochem hypothetical grain Ilford Ilford L4 image spread immunoglobulin isotopes Kodak NTE labelled lectins lipid magnification mean measurements membrane methods in electron Microdol micrographs microscope mitochondria molecules nuclear obtained organelle osmium tetroxide particles particular peroxidase phosphate buffer Poisson distribution precursor preparation protein quantitative radioactive radiochemical radioisotope reagents Rinse saccharide Salpeter sample section thickness silver grains slide sodium solution specimen staining stereological surface synthesis Table technique tissue tritiated tritium ultrastructural ultrathin sections volume Wash Weibel Williams