Academic Press, Sep 21, 2011 - Science - 432 pages
This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques.
*fully revised second edition
*organized into basic and advanced Methods
*new section on Functional Genomics
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analysis antibody assays avian embryos binding sites Biol blastoderm Bronner-Fraser buVer cDNA Cell Biology cell death chick embryo chicken chimeras conserved coverslip culture detection developmental diluted dissecting diVerent diVerentiation diYcult donor dorsal Douarin ectoderm EGFP electrode electroporation enhancer epiblast ethanol eVects explants eYcient filter fluorescent protein forceps gene expression genome graft host hybridization Incubate injection insert KTBT Kulesa labeled limb bud macroarray markers mesenchyme mesoderm Methods in Cell microscope migration molecular morpholino mouse needle neural crest cells neural plate neural tube neurons notochord photoactivation pipette plasmid Preparation primer primitive streak probe Protocol quail regulatory elements removed Ringer’s solution room temperature RT-QPCR sections sequence signal slices somites Sox2 stage staining sterile suYcient target technique time-lapse imaging tissue transcription factor transfection transgenic transplantation trigeminal vectors ventral vertebrate vitelline membrane vitro vivo Volume Wash yolk