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The Roots and Shoots of Molecular Genetics
DNA Recombination in Plasmids and the Chromosome
A Genetic System for Stabilization of Cloned Genes
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0gasawara 1986 by Academic 35 regions a-amylase Acad Academic Press activity alkaline phosphatase amino acid amino acid sequence amylase analysis antibiotic assay BACILLUS MOLECULAR GENETICS BACILLUS SUBTILIS bacitracin Bacterid Bacteriol BamHI Biochem Biol BIOTECHNOLOGY APPLICATIONS chloramphenicol chromosome cloned constructed containing crystal protein culture deletion digestion DNA fragments DNA sequence DnaA encoded enzyme expression form reserved gene product Hindlll Hoch homology host Hulett hybrid indicated initiation insertion isolated ISP-I licheniformis located Losick marker medium membrane Microbiol mRNA mutants Natl nucleotide nucleotide sequence open reading frame operon peptide phage phenotype plasmid polypeptides Proc promoter protease protein protoplasts pUBH0 recombinant replication rights of reproduction RNA polymerase SASP genes secretion vector shown in Figure signal sequence specific spo0A spo0F gene spore sporulation stem-loop strain strand streptomycete subtilisin Sueoka synthesis transcription transformation transposon upstream vector vitro vivo Yoshikawa