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REVIEW OF THE LITERATURE
METHOD AND MATERIALS
RESULTS OF INVESTIGATION
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24 hours acid acrylic aerobically anaerobic jar bacteria bacteriostatic brain heart infusion calcium hydroxide carbide bur Cavaseal cavity preparation Clay-Adams clinical contained Copalite varnish culture medium culture tubes Dent dental restorations dentin shavings dentino-enamel junction disks recovered disks were recovered drops of varnish edsu eth fo evaluated extracted teeth filter paper disks floor gentian violet glucose ascites medium human mouth incubated aerobically inoculated isotopes lined with S. S. lined with varnish marginal leakage marginal penetration Massler mesial methylene blue Micrococcus tetraganes oral cavity organisms percent permeability Petri dishes phosphoric acid plates preventing penetration prior to placing produced growth produced positive cultures removed Rubber dam S. S. White cavity samples showed growth silicate cement silicate restorations silver amalgam restorations Staphylococcus Staphylococcus epidermidis sterile Streptococcus vlrldans surface thiw tions tooth structure varnish showed varnish-lined amalgam restorations week Wesco White cavity liner zinc phosphate cement