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2-litre flask acetic acid Adjust allow anaerobic animal Apparatus Required aqueous solution Bacillus bacteria blood boiling bottle bouillon broth Bunsen burner c.c. capacity c.c. distilled water c.c. sterile capillary capsule cells cent chloride colonies colour containing cool cover-slip cubic centimetre cultivation culture cylinder dilution dish disinfectant distilled water drop emulsify emulsion Erlenmeyer flask examination film filtrate fluid forceps funnel gelatine gentian violet glass tubing glucose grammes growth hanging-drop heat hydrogen incubate at 370 infected inoculation laboratory liquefied litmus litre Measure meat extract medium method micrometer microscope milk mixture NaOH needle nutrient agar nutrient bouillon nutrient gelatine organism peptone phenol piece plate Prepare quantities reaction Remove rubber sample serum slide sodium spores stain steam steamer at 1oo sterile sterile pipettes sterilise stopper surface surface plates syringe temperature test-tube tion tissue twenty minutes vide water-bath Weigh xylol