Bacteriophages: Methods and Protocols, Volume 2: Molecular and Applied Aspects

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Martha R. J. Clokie, Andrew Kropinski
Humana Press, Dec 15, 2008 - Science - 373 pages
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Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular BiologyTM series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

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Contents

Preparation of Bacteriophage Lysates and Pure
3
Approaches to the Compositional Analysis of
11
Determination of Bacteriophage Genome Size by PulsedField
19
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