Basic Methods in Microscopy: Protocols and Concepts from Cells : a Laboratory Manual

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David L. Spector, Robert D. Goldman
CSHL Press, 2006 - Science - 382 pages
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Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory. Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events. This manual is an important tool for any biology researcher employing imaging as a research method.
 

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Contents

Ch 1 Light Microscopy
1
Ch 2 Confocal Microscopy Deconvolution and Structured Illumination Methods
43
Ch 3 Multiphoton and Multispectral Laserscanning Microscopy
83
Ch 4 Preparation of Cells and Tissues for Fluorescence Microscopy
105
Ch 5 Nonimmunological Fluorescent Labeling of Cellular Structures
125
Ch 6 Introduction to Immunofluorescence Microscopy
145
Ch 7 Immunostaining of Microtubules Microtubuleassociated Proteins and Intermediate Filaments
155
Ch 8 Immunofluorescence Localization of Actin
165
Nonisotopic Labeling
213
Ch 15 Fluorescence In Situ Hybridization to DNA
223
Ch 16 WholeMount Fluorescence In Situ Hybridization to Drosophila Chromosomal DNA
261
Ch 17 In Situ Hybridization to RNA
277
Ch 18 WholeMount In Situ Detection of RNAs in Vertebrate Embryos and Isolated Organs
291
Ch 19 Image Production Using Transmission Electron Microscopy
303
Ch 20 Preparative Methods for Transmission Electron Microscopy
307
Ch 21 Immunoelectron Microscopy
343

Ch 9 Immunofluorescence Localization of Nuclear Proteins
171
Ch 10 Immunofluorescence Methods for Saccharomyces cerevisiae
175
Ch 11 Immunofluorescence Methods for Drosophila Tissues
183
Ch 12 Immunofluorescence Methods for Caenorhabditis elegans
193
Nonisotopic Labeling
201
Lenses Filters and EmissionExcitation Spectra
359
Appendix 2 Cautions
378
Basic Micro Index
379
Back cover
383
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