Basic Techniques in Biochemistry and Molecular Biology
Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.
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pH and Buffers
Centrifugation and Cell Fractionation
Amino Acids and Proteins
Vitamins Hormones and Pigments
30 minutes absorbance add 2 ml adsorption agarose alcohol Alkaline amino acids amylose anthrone antibody antigen Basic Techniques Biochemistry and Molecular blank blue boiling water bath Calculations Carbohydrates carbonate cells centrifuge centrifuge tube change in colour chloride chromatography coloured complex column compounds concentration Cool dilute Dissolve distilled water electrophoresis ELISA elution enzyme Estimation ethanol EXPERIMENT extract filter paper filtrate flask fraction furfural given in Table glucose heat homogenate Incubate layer lipid litre mg/ml Mix the contents mixture ml of distilled mmol/litre Molecular Biology molecular weight molecules NaOH Ninhydrin nm wavelength particles pellet perchloric acid phenol pipette potassium Prepare Procedure protein pyruvate reaction reacts Reagents ml Requirements room temperature sample sedimentation separation serum sodium carbonate Sodium hydroxide solvent system spectrophotometer standard curve sucrose sulphate sulphuric acid supernatant Techniques in Biochemistry TEST Principle test tubes tissue titration trichloroacetic acid tryptophan volume wash