Basic Techniques in Biochemistry and Molecular Biology

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I. K. International Pvt Ltd, Nov 15, 2008 - Biochemistry - 280 pages
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Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.
 

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Contents

Laboratory Safety
1
pH and Buffers
8
Spectrophotometry
15
Contents
22
Contents
24
Centrifugation and Cell Fractionation
28
Chromatography
39
Paper Chromatography
56
Amino Acids and Proteins
105
Nucleic Acids
132
Electrophoresis
142
Immunology
154
Enzymes
163
Metabolites
185
Vitamins Hormones and Pigments
208
Molecular Biology
215

Carbohydrates
66
Lipids
92
Appendices
251
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