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Preparation of Tissue Blocks
Rinsing Dehydration and Embedding
Procedure for Minimizing Chemical Hazards during
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0.1 M cacodylate acetone acid acrolein added agar aldehyde allowed aqueous Araldite BEEM capsule block face buffer pH 7.2 CaCl2 cacodylate buffer pH carbon films cells centrifuged coated concentration coverslip cutting edge dehydration diameter distilled water drop droplets electron microscopy Epon ethanol evaporation filter paper fixation fixative solution floated formaldehyde Formvar film glass knife glass slide glutaraldehyde glutaraldehyde in 0.1 grids holder hr at 4°C hr at room immersed infiltration layer lead citrate lipid medium mica mosmols NaOH negative stain Osmium osmolality OsO4 overnight Pasteur pipette pellet perfusion Petri dish phosphate buffer pH piece pipette placed plastic polymerization postfixed poststained prepared procedure propylene oxide removed resin room temperature Semithin sections sodium specimen block specimens are fixed specimens are postfixed staining solution stock solution sucrose support film surface suspension syringe thick Thin sections tissue blocks transferred trimmed trough tube ultrathin sections uranyl acetate vial virus washed