Binding constants: the measurement of molecular complex stability
This unique book presents a systematic review of the methods for the determination of binding constants of complex formation in solution. Collects material that has been scattered throughout the literature of several separate fields. Offered here are methods from the areas of acid-base chemistry, metal-ion coordination compounds, hydrogen-bonding, charge-transfer complexation, hydrophobic interaction, and protein-binding. Discusses the relevant thermodynamics, modelling, statistics and regression analysis, and interpretation of data. Includes fresh discussions of random association (contact complexes), selection of standard states, and comparison of results. Treats all of the experimental methods useful for measuring these equilibrium constants, including those based on spectrophotometry, nuclear magnetic resonance, reaction kinetics, potentiometry, solubility, liquid-liquid partitioning, dialysis, chromatography, flourimetry, and many others.
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PART ONE General Matters
Statistical Treatment of Data 103
9 other sections not shown
1:1 binding 1:1 complex a-cyclodextrin according to Eq acid Acta activity coefﬁcient Anal analysis applicable aqueous binding constants binding isotherm Biochem calculated Chem chemical Chromatogr chromatographic compartment complex formation complex SL concentration scale curve cyclodextrin deﬁned deﬁnition described dialysis difﬁculty dissociation double-reciprocal effect enzyme kinetics equilibrium constant equilibrium dialysis evaluated example experimental ﬁeld Figure ﬁnd ﬁrst ﬂuorescence free ligand concentration function interaction inverse matrix isotherm K. A. Connors kinetics least-squares macromolecule mass balance matrix measured membrane method micelle molar absorptivity molecular molecule nonlinear regression normal equations observed obtained partition coefﬁcient Pharm Phys plot according plotting forms quantity ratio rectangular hyperbola Scatchard plots Section self-association signiﬁcant slope solubility solution solvent species speciﬁc spectrophotometric spectroscopy stability constants stationary phase stoichiometric substrate substrate and ligand substrate concentration Table technique tion treatment University of Wisconsin-Madison values variables x-reciprocal yields