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Identification and solution structure of a highly conserved Cterminal
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50 mM phosphate acid chaperone activity affinity RNA binding amino acid binds RNA Boeke Branciforte buffer carboxy central line width Chapter chemical shift chemical shift perturbation coiled-coil component cysteine cysteine mutants determined dihedral angles dynamics encoded endonuclease EPR spectra essential for retrotransposition Figure filter binding assay fold fragment full length ORFlp function Genet genome helix high affinity RNA highly conserved Hill coefficient HSQC Interspersed Nuclear Element-1 Kazazian L1 retrotransposon L1 RNA Long Interspersed Nuclear Martin Md-A Moran motif motif X mouse ORFlp mutagenesis mutants nucleic acid nucleic acid chaperone nucleotide ORF1 ORF1p ORF2p ORFl protein ORFlp presence of RNA Proc Natl Acad protease sensitive linker proteolysis protomer region Reson retrotransposition retrotransposition activity reverse transcriptase reverse transcription Rl nitroxide RNA binding RNA binding affinity RNA binding properties RNA binding surface RNA molecule SDSL-EPR side-chain stoichiometry structure of ORFlp studies transcription trimer unstructured wild-type