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Alteration of Ribosomes Induced by Colicin
f In vitro incorporation
h Sucrose density gradient centrifugation
3 other sections not shown
23s core acid activity added adsorbed adsorption amount of colicin assay biochemical effects Biol centrifugation chloramphenicol coli colicin action colicin E3 colicin Ib colicinogenic cells concentration containing control and E3 control ribosomes cts/min described in Materials dialyzed dinitrophenol E3-inactivated ribosomes E3-treated cells Effect of colicin Figure filters formamide formamide-treated gradient sedimentation analysis homologous colicin Ib-colicinogenic immune cells immunity breakdown inactivated ribosomes inactivation of ribosomes incorporation incubation inhibition of protein isolated from E3-treated killing units Materials and Methods membrane messenger RNA minutes mixture Nomura and Maeda non-colicinogenic non-nucleotide organic phosphate Optical density organic phosphate fractions phage phenylalanine phosphate Poly U dependent polyamine polysomes protein synthesis putrescine radioactivity receptor ribosomal RNA ribosome inactivation ribosomes isolated sample sensitive cells shows specific split protein strain N049 sucrose gradient centrifugation Sucrose gradient sedimentation supernatant Table TB medium TMM buffer tRNA trypsin tryptone tube ug/ml vivo washed