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7-glutamyl a-ketoglutarate acidotic amino acids ammonia production animals antibody aspartate ATPase basal basement membrane bicarbonate Biochem brane brush border brush border membrane calcium cell changes chloride citrate collagen collecting duct concentration connecting tubule cortical cyclic AMP D-glucose decreased distal effect enzyme enzyme activity excretion experiments fatty acids flux fraction glucagon gluconeogenesis gluconeogenic glucose glucose production glutamate glutaminase glutamine transport gradient Guder hormone increase incubation infusion inhibition intracellular ionophore K-ATPase kidney cortex kidney mitochondria kidney tubules labelled lactate lipid liver measured medium membrane vesicles metabolic acidosis microvillus mitochondria nephron nucleotide ophthalmic acid ouabain oxidation oxygen consumption palmitate pathway peptidase peptides perfused phosphate plasma membrane present protein proximal convoluted proximal tubule pyruvate rabbit rectal gland renal gluconeogenesis segments serum shown slices solution specific stimulation studies substrate synthesis synthetase Table tion tissue tubular uptake urine vitro vivo